![]() However, a better choice for multiple labeling is Rhodamine Red-X because its fluorescence is midway between a green fluorescing dye (like Alexa Fluor 488) and a far-red-fluorescing dye like Alexa Fluor 647. C圓 can also be paired with Alexa Fluor 647 for multiple labeling when using a confocal microscope. C圓 has been used with fluorescein for double labeling however, the use of a narrow band-pass emission filter for fluorescein is recommended to minimize C圓 fluorescence in the FITC filter set. Goat anti Chicken IgG (Fc) antibody recognizes the heavy chains of chicken IgG, specifically, epitopes within the Fc region and shows no cross-reactivity. C圓 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). ![]() We recommend C圓 as a brighter alternative to TRITC. For fluorescence microscopy, C圓 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background. C圓 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. To minimize cross-reactivity, the goat anti-chicken IgY whole antibodies have been affinity-purified and cross-adsorbed against chicken serum containing non-immunoglobulin chicken serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but it may cross-react with immunoglobulins from other species.Ĭ圓 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates. No antibody was detected against non immunoglobulin serum proteins. It also reacts with the light chains of other chicken immunoglobulins. Freund’s complete adjuvant is used in the first step of the immunization procedure.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY. while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies. Purified Fab of normal IgG isolated from pooled chicken serum. Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody. In precipitating techniques as immunoelectrophoresis and single and double radial immuno- diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins as secondary antibody to precipitate the immunoglobulin in normal chicken serum. Precipitating polyclonal goat antiserum to chicken Fab of IgG. It will not affect the performance of the product. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Working dilutions should be stored at +4☌, not refrozen, and preferably used the same day. Repeated thawing and freezing should be avoided. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). The lyophilized antiserum is shipped at ambient temperature and may be stored at +4☌ prolonged storage at or below -20☌. In double radial immunodiffusion use a rosette arrangement with 10 ulantiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Cross- reactivity of this antiserum has not been tested in detail.IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. Bovine, Goat, Guinea pig, Horse, Human, Mouse, Rabbit, Rat, Sheep. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat seru No foreign proteins added.ĭelipidated, heat inactivated, lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum.
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